Sari Edi Cahyaningrum, Narsito, Sri Juari Santoso, Rudiana Agustini
Chitosan beads were prepared by using a cross-linking agent Cu(II) and Zn(II) metal ions and the metal ion- chitosan beads were employed in papain immobilization processes. Studies on free and immobilized papain systems for determination of optimum pH, optimum temperature, thermal stability and reusability were carried out. The results showed that free papain have been optimum pH 6,5 and optimum temperature 55°C while the immobile papain had optimum pH 8 and optimum temperature 85°C. The thermal stability of the immobilized papain, relative to that of the free papain, was markedly increased. The residual activity of papain immobilized on chitosan bead- metal ion was about 25% after 12 cycles of batch operation.
Department of Chemistry, Surabaya State University, Indonesia; Department of Chemistry, Gadjah Mada University, Indonesia
