Nuniek Herdyastuti, Sari Edi Cahyaningrum, Tri Joko Raharjo, Mudasir, Sabirin Matsjeh
Bacteria isolated from mud field in the Ketintang Surabaya area showed chitinolytic activity in media containing 0,4 % colloidal chitin. Morphological and phisiological characteristic showed Gram negative, rod-shape kokoid, can produce acid from mannitol, sucrose, sorbitol, innositol also able to oxidize glucose. Based on the nucleotide sequence of 16S-rRNA gene showed 98 % genetic relationship to Pseudomonas sp. Molecular weight chitinase determination by SDS-PAGE and zymogram have size 26.1 and 29.0 kDa. Extraselluler chitinase can showed inhibition of growth of Aspergillus parasiticus fungi that are pathogenic on rice and potatoes.
Department of Chemistry, Surabaya State University, Ketintang Surabaya, Indonesia; Department of Chemistry, Gadjah Mada University, Sekip Utara Yogyakarta, Indonesia
