Sari Edi Cahyaningrum, Maria Monica Sianita
Glutaraldehyde crosslinked chitosan - silica nanobeads have been used as a support in the immobilization process of pepsin. The effect of pH, pepsin concentration, and temperature on the characteristics of both free and immobilized pepsin had been investigated in this study. The results showed that the immobilized pepsin has wider pH range, better capacity, performance and stability to high temperatures compared with those of free pepsin. The immobilized pepsin can be used for 8 to 10 times at which the activity remains 20%, whereas the free pepsin can only be used once. These results suggested promising applications of immobilized enzyme as biocatalyst. © 2014 BCREC UNDIP. All rights reserved.
Department of Chemistry, Faculty Mathematics and Natural Science, State University of Surabaya, Jl. Ketintang Surabaya, 60231, Indonesia
