Yuliani, Soemarno, Bagyo Yanuwiadi, Amin Setyo Leksono
The diversity of secondary metabolites in plants, in general, can caused by genetic factor or environment which is interconnected. It can occur because of differences in population, life cycle, cultivation time, soil, climate, and geographic condition. The aim of the study was to quantitatively analyze the phenolic and flavonoid content of Pluchea indica leaves extract from different altitude habitats. Pluchea indica leaves were obtained from three different altitude habitat: lowland (Bangkalan; 28.3 - 31.72 m), middleland (Trawas; 727 – 937 m) and highland (Batu; 1303 – 1322 m). The simplicia of P. indica leaves were macerated and extracted with methanol, ethyl acetate, aquades and n-butanol. The total phenolic (gallic acid/GAE) and flavonoid (quercetin/QE) contents were determined using UV-VIS spectrophotometer. The results showed that the total phenolic contents of lowland (1,763 ±0,047 mg/mL) was found higher as compared to the middle-altitude land (1,455 ±0,295 mg/mL) and highland (1,212 ±0,608 mg/mL). The total flavonoid contents of P. indica were showed no mean difference of lowland (3,1 mg/mL), middle-altitude land (3.0 mg/mL) and highland (3.2 mg/mL). The highest phenolic and flavonoid content was found to be in ethyl acetate fraction of P. indica. © 2015, Sphinx Knowledge House. All rights reserved.
Department of Biology, Brawijaya University, Indonesia; Surabaya State University, Indonesia; Brawijaya University, Indonesia
