Sari Edi Cahyaningrum, Nuniek Herdyastusi, Dina Kartika Maharani
In this study, the macroporous chitosan beads was prepared, and could be obtained by chelating Cu2+, Zn2+, Ca2+, and Mg2+ ions, respectively on chitosan beads, and Glucose Isomerase (GI) could be adsorbed on the Chitosan beads-metal ions adsorbent through metal-protein interaction forces. Batch adsorption experiments show that adsorption capacity for GI on these chitosan beads-metal ions adsorbent varieties with change of pH. The effect of chelated metal ions species on chitosan beads on the activity and stability of immobilized GI was also evaluated and discussed. Chitosan beads-Zn was the best adsorbent among four kinds of Chitosan beads- metal ions for GI immobilization since it has high enzyme loading, high stability and activity retaining the macroporous metal chelated bead could be pervasively applied in enzyme adsorption or immobilization provided the enzyme had metal chelating ability.
Department of Chemistry, Surabaya State University, Indonesia
