N. Herdyastuti, S.E. Cahyaningrum
N-Acetylglucosamine is the monomeric unit of the polymer chitin with (1,4)-β-linkages and the second most abundant carbohydrate after cellulose. The purpose of this study was to analyze N-acetyl glucosamine from enzymatic degradation chitin. The method used is the enzymatic hydrolysis of chitin types amorphous using chitinase from Pseudomonas sp. TNH54. Chitin is a kind of amorphous modified chitin and showed high activity against chitinase. N-acetylglucosamine production optimization has been done with a substrate concentration of 1.2%, enzyme concentration of 0.097 U/mL and the incubation time of 8 hours. The result of purification with ethanol shows a peak with a retention time of 3.1 and a purity of 96.7%. The result showed that the N-acetyl glucosamine hydrolysis enzymatic is in accordance with the standards. Furthermore, experiments to establish purification of N-Acetylglucosamine by ethanol - acetonitrile provides a fairly high yield with similar properties commercial N-Acetylglucosamine. © RASĀYAN. All rights reserved.
Department of chemistry, Universitas Negeri Surabaya, Jl. Ketintang, Surabaya, 60231, Indonesia
