F. Rachmadiarti, G. Trimulyono
The effect of lead (Pb) pollution on water clover resistance was determined by the Pb distribution and the percentage of free amino acids in plant tissues. Water clover plant from Surabaya, Indonesia was collected and plants of equal size were grown hydroponically. They were exposed into 0, 1, 5, 10 mgL-1 of Pb concentration for 10, 20, and 30 days. The experiment was a randomized completely block design with 3 replications. The Pb concentration was measured by inductively coupled plasma (ICP) and the free amino acid was analyzed by HPLC (High-Pressure Liquid Chromatography). The Pb concentration in plant organs and its free amino acids content indicated that water clover plant was resistance on Pb accumulation and affected their growth which potential for phytoremediation. The highest concentration of Pb was found in root tissue, followed by stem and leaf tissues. These findings indicated the respond of water clover plants resist on Pb that determined by the increased percentage of free amino acids and phytochelatins such as glutamic acid, cysteine, glycine, and proline. © 2019, ALÖKI Kft., Budapest, Hungary.
Faculty of Mathematics and Sciences, Universitas Negeri Surabaya, Jalan Ketintang, Surabaya, 60123, East Java, Indonesia
