Anisya E. Juniar, Reni Ambarwati, Dwi A. Rahayu
Clithon oualaniense is a member of the Neritidae (Mollusca: Gastropoda), which has many shell variations, hence it often causes erroneous morphological identifications. The DNA barcode provides a molecular taxonomy solution by using short DNA sequences to identify species. This study aimed to characterize the DNA barcode of C. oualaniense and to analyze the phylogenetic relationship of C. oualaniense from Madura, Indonesia. Samples were taken from Labuhan Beach Bangkalan Madura using a purposive sampling method by taking three dominant variations and two non-dominant variations, then preserved in absolute ethanol. The molecular characterization was performed using Cytochrome Oxidase subunit I (COI) markers, through: DNA isolation, DNA amplification (using COI barcode primers), electrophoresis and genetic analysis (using a bioinformatics software). The genetic identification was carried out using research sample data and comparison gene data from GenBank NCBI. The COI DNA barcoding resulted in a DNA sequence length of 667 bp consisting of 10 types of transition mutations and 657 of conserved nucleotide bases. The phylogenetic tree reconstruction of the C. oualaniense research samples from GenBank, through the Neighbor-Joining and Maximum Likelihood methods, formed one cluster with a bootstrap value of 100, with a similarity value between 98.26-100%. The analysis using COI barcode DNA markers has successfully identified the species C. oualaniense from Madura, Indonesia. © 2021, BIOFLUX SRL. All rights reserved.
Biology Department, Faculty of Mathematics and Natural Sciences, Universitas Negeri Surabaya, Jln. Ketintang, Gayungan, Surabaya, 60231, Indonesia
