Dyah Hariani, Dwi Anggorowati Rahayu, Pungky Slamet Wisnu Kusuma
The African sharptooth catfish (Clarias gariepinus) has received widespread attention due to increase of global demand. However, availability of C. gariepinus breeding depends on the season, especially during the spawning season. The objective of this study was to investigate the effect of soft laserpuncture induction on testosterone level, Androgen Binding Protein (ABP) level, and numbers of Sertoli and Leydig cells of male C. gariepinus. An approximately one-year old virgin male fish was selected based on the healthiness and the body weight of 1,000-1,200 g. Fishes were treated by laserpuncture induction every 15 days from 0 to 75 days. The food containing 38% crude protein was used for feeding procedure. This study adopted a completely randomized design. The parameters observed were the levels of testosterone, ABP, and numbers of Sertoli and Leydig cells measured at 0, 15, 30, 45, 60, and 75 days after treatment. The Sertoli and Leydig cell determinations were analyzed descriptively, whereas the testosterone and ABP levels were evaluated using analysis of variance. The results indicated that induction of laserpuncture improves male catfish reproductive ability through enhancement of the testosterone level, ABP level, and numbers of Sertoli and Leydig cells. The testosterone level, ABP level, and numbers of Sertoli and Leydig cells increased and peaked on the30th and 60th days, indicating a cycle of higher spermatozoa quality every 30 days. © 2021, Egyptian Society for the Development of Fisheries and Human Health. All rights reserved.
Department of Biology, Universitas Negeri Surabaya, Ketintang Campus, Jalan Ketintang, C3 Building, Surabaya, 60231, Indonesia; Universitas PGRI Adibuana, Jl. Dukuh Menanggal XII 4, Surabaya, Indonesia
