Suci Y. P. Sari, Reni Ambarwati, Dwi A. Rahayu
Donax faba highly varies in the pattern and color of the shell, but there is no consistent type of variation. This morphological variation needs to be reviewed based on genetic characteristics. The molecular marker of the cytochrome oxidase subunit I (COI) gene is a genetic marker with a high genetic variation. This study aimed to identify the characteristics of DNA barcodes of Donax faba from Nepa Beach, Madura, Indonesia. Donax faba samples were collected from Nepa Beach, Madura, Indonesia, using a purposive sampling method by selecting three dominant types of variation and two types that were less dominant. The samples obtained were preserved in absolute ethanol. Molecular identification was carried out by the stages of DNA isolation, amplification using COI universal primers, electrophoresis, sequencing, and genetic analysis using bioinformatics software. The results were compared with data from the GenBank. The results of DNA barcode identification using the COI gene presented a sequence of 650 bp with a similarity value of 72.01-72.12%. The Donax faba COI gene sequence has a high mutation rate which causes a high genetic variation resulting in genetic differentiation between Donax faba from Nepa Beach and Donax faba from Thailand. Thus, differences in geographic location can affect the genetic plasticity of Donax faba. © 2021, BIOFLUX SRL. All rights reserved.
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Surabaya, Jln. Ketintang, Gayungan, Surabaya, 60231, Indonesia
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