Salamun, Rizky Danang Susetyo, Ni’matuzahroh, Fatimah, Almando Geraldi, Agus Supriyanto, Tri Nurhariyati, Farah Aisyah Nafidiastri, Nabilatun Nisa’, Endarto
Biosurfactants as biocontrol agents have received much attention for pest control and disease vectors. The research aimed to identify the species and genetic relationship, hemolytic activity, detect coding genes, and trial production of biosurfactants on various substrates of entomopathogenic Bacillus sp. BK7.1 isolated from natural soil in Baluran National Park, East Java, Indonesia. Biosurfactant screening was carried out by testing hemolytic activity, surface tension, and emulsification activities, detecting coding genes of biosurfactant biosynthesis, and testing biosurfactant production in various substrates. The results of the molecular identification by amplifying the 16S rRNA gene using the Polymerase Chain Reaction (PCR) method for Bacillus sp. BK7.1 has a genetic similarity of 98.68% with B. subtilis subsp. inaquosorum strain BGSC 3A28. Screening showed positive hemolytic activity results, reduced surface tension, increased emulsification activities, and the production of biosurfactant in glucose, glycerol, and molasses substrates. The PCR results showed that Bacillus sp. BK7.1 had srfAA and srfAD genes encoding surfactin biosynthesis, giving it the potential to produce bioinsecticide compounds. Based on these studies, the indigenous entomopathogenic B. subtilis BK7.1 can be developed as environmentally friendly microbial bioinsecticides for pest control and disease vectors. © 2023, Society for Indonesian Biodiversity. All rights reserved.
Laboratory of Microbiology, Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Kampus C, Jl. Dr. Ir. H. Soekarno, Mulyorejo, East Java, Surabaya, 60115, Indonesia; Research Group for Applied Microbiology and Bioresource Technology, Universitas Airlangga, Jl. Dr. Ir. H. Soekarno, Mulyorejo, East Java, Surabaya, 60115, Indonesia; University of Co-E-Research Center for Bio-Molecule Engineering, Universitas Airlangga, Jl. Mulyorejo Surabaya, East Java, Surabaya, 60115, Indonesia; Laboratory of Entomology, Institute of Tropical Diseases, Universitas Airlangga, Jl. Dr. Ir. H. Soekarno, Mulyorejo, East Java, Surabaya, 60115, Indonesia; Institute of Life Science, Technology and Engineering, Universitas Airlangga, Kampus C, Jl. Dr. Ir. H. Soekarno, Mulyorejo, East Java, Surabaya, 60115, Indonesia; Laboratory of Microbiology, Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Surabaya, Jl. Ketintang, East Java, Surabaya, 60213, Indonesia; Laboratory of Molecular Genetics, Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Jl. Dr. Ir. H. Soekarno, Mulyorejo, East Java, Surabaya, 60115, Indonesia; Research Development, Baluran National Park. Jl. Raya Banyuwangi-Situbondo Km 35, Situbondo, East Java, 68374, Indonesia
