Ahmad Rudi Setiawan, Syananda Zahra Fadila, Teguh Hari Sucipto, Shifa Fauziyah, Safira Madaniyah, Eryantika Cipta Dewi, Sin War Naw, Tukiran, Sari Edi Cahyaningrum
Aedes sp. is a carrier of several viruses that can infect humans and cause diseases such as zika, yellow fever, chikungunya, and dengue fever. Symptoms of dengue infection vary, consisting of classic dengue fever (DD), dengue hemorrhagic fever (DHF), and dengue shock syndrome. Insecticide spray can be used to manage Aedes mosquitoes chemically. Insecticide substances target nervous system proteins. Voltage-gated sodium channels (VGSC) are rendered inactive by pyrethroid binding. Knockdown is a signal indicating an insect has been knocked down in response to a specific insecticide. However, using insecticides for a long time can cause mosquitoes to become resistant. The pesticide resistance of mosquitoes is known as knockdown resistance (kdr). This study aims to detect kdr mutations (V1016G) in two male Aedes albopictus mosquitos named A1 and A2 collected from settlements in Kranggan, Sawahan, Surabaya, East Java, Indonesia, using allele-specific polymerase chain reaction (AS-PCR) assay. RNA was extracted from the two mosquito samples using an RNA extraction kit. After that, the extracted RNA was tested for kdr mutations using the AS-PCR method. After assaying, both samples are homozygous wildtype (V1016V) because the results showed bands appearing from samples A1 and A2 at 60 bp. On the other hand, this study has the potential to serve as preliminary monitoring for the program controlling vectors. © 2023, Society for Indonesian Biodiversity. All rights reserved.
Department of Chemistry, Faculty of Mathematics and Natural Science, Universitas Negeri Surabaya, Jl. Ketintang, Gayungan, East Java, Surabaya, 60231, Indonesia; Dengue Study Group, Institute of Tropical Disease, Universitas Airlangga, Jl. Dr. Ir. H. Soekarno, Mulyorejo, East Java, Surabaya, 60115, Indonesia; Akademi Analisis Kesehatan Delima Husada, Jl. Arif Rahman Hakim No. 2B, Kramatandap, Gapurosukolilo, Gresik, East Java, 61111, Indonesia; MBKM Program, Research Center on Global Emerging and Re-emerging Infectious Diseases, Institute of Tropical Diseases, Universitas Airlangga, Jl. Dr. Ir. H. Soekarno, Mulyorejo, East Java, Surabaya, 60115, Indonesia; Department of Chemistry, Myitkyina University, Sitapu Quarter, Mytkyna Township, Myanmar
