D. Yuliastrid, N.W. Kusnanik, B. Purwanto, A. Noordia, S.P. Purwoto, A. Pranoto
Endurance was essential to perform an activity for as long as possible. Previously, haemoglobin, myoglobin, lactate and interleukin (IL)-6 level played a key role in determining endurance. This study aims to analyse the differences in the duration of running a treadmill on the levels of haemoglobin, myoglobin, lactate, and IL-6 in mice (Mus musculus). The mice were forced to run on the treadmill for as long as possible. They were divided into 3 groups, namely Short (n = 12; ≤12.5 min treadmill running), Moderate (n = 12; 12.5-40 min treadmill running), and Long (n = 12; ≥40 min treadmill running). Haemoglobin and lactate levels were evaluated, respectively, using the Quick-check Plus Hb Haemoglobin Testing System and Accutrend® Plus system. Analysis of myoglobin and IL-6 levels used an enzyme-linked immunosorbent assay (ELISA) kit. Statistical analysis used the one-way ANOVA, and LSD post-hoc tests with a significance level of 5%. The results showed that only the myoglobin level characterised endurance. Higher myoglobin levels were found at longer duration of running. Haemoglobin, lactate and IL-6 levels did not differ between groups. Based on the results of the study it was concluded that a longer duration of running was found a higher level of myoglobin. Myoglobin but not haemoglobin represented as an essential characteristic for endurance among runners in mice. High-affinity binding between myoglobin and oxygen was essential for oxygen storage in muscle. © 2023 Brill Wageningen Academic. All rights reserved.
Doctoral Program of Sport Science, Postgraduate Program, Universitas Negeri Surabaya, Lidah Wetan Street, East Java, Surabaya, 60213, Indonesia; Department of Health and Recreation Education, Faculty of Sport Science, Universitas Negeri Surabaya, Lidah Wetan Street, East Java, 60213, Indonesia; Department of Sport Coaching Education, Faculty of Sport Science, Universitas Negeri Surabaya, Lidah Wetan Street, East Java, Surabaya, 60213, Indonesia; Department of Medical Physiology and Biochemistry, Faculty of Medicine, Universitas Airlangga, Mayjen Prof. Dr. Moestopo No. 47 Street, East Java, Surabaya, 60132, Indonesia; Study Program of Physical Education, STKIP PGRI Bangkalan, Soekarno Hatta No. 52 Street, East Java, Bangkalan, 69116, Indonesia; Doctoral Program of Medical Science, Faculty of Medicine, Universitas Airlangga, Mayjen Prof. Dr. Moestopo No. 47 Street, East Java, Surabaya, 60132, Indonesia
