Endik D. Nugroho, Reza Ardiansyah, Nia Kurniawan, Widodo, Dwi A. Rahayu
Echinoderms are marine water invertebrates with diverse morphologies and grouped into five classes, but there are currently limited reports on the species from Gili Ketapang Island, East Java. Therefore, this study aims to identify these species, based on their morphologic and molecular characteristics and to determine the phylogenetics of echinoderms from Gili Ketapang Island. DNA barcodes, based on fragments of the 5' end of the cytochrome c oxidase subunit I gene (COI), have been used to support the morphological examination for the echinoderms’ evolutionary lineages determination. A total of 16 echinoderm samples were collected and identified based on morphological characteristics. Subsequently, molecular characterization and identification were conducted using 503 bp of COI, by considering the gene similarity, sequence variation, genetic distance, phylogenetic topology, based on the Barcode of Life Data (BOLD) System. According to the results, the samples were identified as Diadema setosum, Macrophiothrix longipeda, Archaster typicus, Echinometra mathaei, Holothuria atra, Linckia laevigata, Bohadschia argus, and Ophiactis savignyi. Each species was associated with a particular DNA barcode cluster and the relationship among all was revealed. Automatic Barcode Gap Discovery (ABGD) analysis showed that the analyzed echinoderms were divided into eight species, and these new records established a sequences reference library for the Island. © 2023, BIOFLUX SRL. All rights reserved.
Institut Teknologi & Sains Nahdlatul Ulama Pasuruan, Warung Dowo, Pohjentrek, Pasuruan Regency, East Java, Indonesia; Department of Biology, Faculty of Mathematics and Natural Sciences, University Brawijaya, Malang, Indonesia; Department of Biology, Faculty of Mathematics and Natural Sciences, Negeri Surabaya University, East Java, Indonesia
