Nafisah Nurul Hapsari, Muhammad Ridho Hafid Kurniawan, Teguh Hari Sucipto, Nur Syamsiatul Fajar, Eduardus Bimo Aksono Herupradopo, Shifa Fauziyah, Alicia Margaretta Widya, Febriana Aquaresta, Sin War Naw
Legionellosis is a respiratory infection caused by Legionella pneumophila, a bacterium that can infect protozoa and human lung cells. The disease can be mild or severe, and sometimes fatal, especially in immunocompromised people. Some types of antibiotics that can be used to treat Legionella disease are macrolides such as rifampicin, azithromycin and clarithromycin, as well as fluoroquinolones such as levofloxacin and moxifloxacin. The aim was to investigate the distribution and prevalence of Legionella in well water. This study collected and tested water samples from a different well water in Magetan Regency, East Java, Indonesia using DNA extraction, two-step PCR methods, and visualization with 1.5% agarose gel in UV transluminator. The results were visualized with QGIS 3.28.6 and compared with other tests. The results showed that none of the eight water samples were contaminated with L. pneumophila compared with positive control (403 bp). © 2023, Airlangga University. All rights reserved.
Undergraduated Program, Department of Chemistry, Faculty of Mathematic and Natural Science, Universitas Negeri Surabaya, Indonesia; Dengue Study Group, Institute of Tropical Disease, Universitas Airlangga, Surabaya, Indonesia; Tropical Disease Diagnostic Center, Institute of Tropical Disease, Universitas Airlangga, Indonesia; Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia; Delima Husada Gresik Health Analyst Academy, Gresik, Indonesia; Department of Clinical Microbiology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia; Palembang Health Laboratory, Palembang, Indonesia; Department of Chemistry, Myitkyina University, Sitapu Quarter, Myitkyina Township, Myanmar
