Isnawati, Lisa Lisdiana, Mahanani Tri Asri, Guntur Trimulyono
Fermetodege is referred to as fermented ruminant feed made from rice bran, corn cobs, and heavy metal-free water hyacinth. Several factors influence both the fermentation process and the quality of the final products, including dominance, diversity, and evenness, of indigenous cellulolytic bacteria species. To increase the quality of fermented feed, there is a need to fully comprehend the influential factors. Therefore, this study aimed to assess factors influencing the biodiversity of cellulolytic bacteria isolated from fermetodege. Cellulolytic activity of the isolates was assessed through observation of growth on the carboxymethylcellulose (CMC) media. Simpson’s species dominance, as well as Shannon-Weiner's diversity and evenness indices were also calculated. The results showed that cellulolytic activity of the bacteria isolates formed a clear zone after soaking the bacteria colonies in 0.1% Congo red and rinsing with 1 M NaCl. The Shannon-Weiner’s diversity index was categorized as medium, with values ranging from 0.6849 to 1.8173. Observation on stable distribution of bacteria species showed evenness index between 0.7778 and 0.9983. Meanwhile, the Simpson’s species dominance ranged from 0.1835 to 0.5082, showing that there was no dominant species. In conclusion, this study showed that fermetodege was a potential source of bacteria isolates and could be used as a fermentation consortium starter. © (2023), (Seameo Biotrop). All Rights Reserved.
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Surabaya, Kampus Unesa Ketintang, Surabaya, 60231, Indonesia
