Ulia Asmaul Khusna, Heni Nur Fadzilah, Muhammad Raihan, Hervanny Putri Pramesti, Elok Najwa Salfiah, Dwi Anggorowati Rahayu, Endik Deni Nugroho, Azman Abdul Rahim, Rusdianto
Previous research has reported a common phenomenon of ambiguous species in the family Gobiidae, including mudskippers, which have highly similar morphology and phenetics but are genetically different, resulting in taxonomic confusion. Inaccurate identification of cryptic species can lead to taxonomic and conservation problems. The problems posed are multiple names for the same species, an increase in biodiversity, an increase in ambiguous diversity, and two genetically distinct species classified into one species due to morphological similarities. Research on the molecular identification of mudskippers using the COI mitochondrial gene as a DNA mini-barcoding marker in Indonesia, especially in East Java, still needs to be completed. This research aimed to identify and analysis of the genetic relationship of mudskipper based on the COI (cytochrome-c-oxidase subunit I) gene. DNA samples were collected from 5 locations on the North Coast of East Java, Indonesia. DNA samples were collected by cutting the pectoral fins of caught mudskipper fish. The pectoral fins were stored in pure-grade ethanol and subjected to DNA extraction, PCR, and sequence processing analysis using bioinformatics. The result shows that four species based on eight samples from different locations of mudskipper fish were found to have similarity values with Periophthalmodon schlosseri, Periophthalmus argentilineatus, Boleophthalmus boddarti, and Boleophthalmus pectinirostris, namely in the range of 98.42-100% genetic similarity. Genetic analysis based on nucleotides showed a site frequency of 66.196%, site parsimony of 24.646%, nucleotide diversity of 0.995, and haplotypes 7 and 11 mutations at the first codon position. Based on the phylogenetic tree topology analysis of the research samples, the bootstrap value range for each Operational Taxonomic Unit (OTU) was between 98 and 100, which indicates that the samples have significant similarities or belong to the same species in one cluster. © 2024, BIOFLUX SRL. All rights reserved.
Biology Study Program, Faculty of Mathematics and Natural Sciences, Universitas Negeri Surabaya, East Java, Surabaya, Indonesia; Biology Education Program, University Nadhlatul Ulama Pasuruan, East Java, Pasuruan, Indonesia; Marine Ecosystem Research Centre (EKOMAR), Faculty of Science and Technology, Kebangsaan Malaysia University, UKM, Selangor, Bangi, Malaysia; Research Center for Biosystematics and Evolution, National Research and Innovation Agency (BRIN), Indonesia; Biologi Education Study Program, Faculty of Mathematics and Natural Sciences, Universitas Negeri Surabaya, Jl. Ketintang, Ketintang, Gayungan Regency, East Java, Surabaya, 60231, Indonesia; Chemistry Study Program, Faculty of Mathematics and Natural Sciences, Universitas Negeri Surabaya, Jl. Ketintang, Ketintang,Gayungan Regency, East Java, Surabaya, 60231, Indonesia; Chemistry Education Study Program, Faculty of Mathematics and Natural Sciences, Universitas Negeri Surabaya, Jl. Ketintang, Ketintang, Gayungan Regency, East Java, Surabaya, 60231, Indonesia; Biology Education Program, University Nadhlatul Ulama Pasuruan, Jl. Raya Warung Dowo Utara Pohjentrek Regency, East Java, Pasuruan, 67171, Indonesia; Marine Ecosystem Research Centre (EKOMAR), Faculty of Science and Technology, Universiti Kebangsaan Malaysia, UKM, Selangor, Bangi, 43600, Malaysia
