Suryanto Agung Prabowo, Junian Cahyanto Wibawa, Hamdani, Nanang Indriarsa, Muchamad Arif Al Ardha, Taufiq Hidayat, Irmantara Subagio, Iwan Barata, Joseph Lobo, Novadri Ayubi
Introduction: Physical exercise has been identified as one of the external factors that can activate AMPK, but the mechanism and effects are still unclear and need to be further explored. Objective: The aim of this study was to examine how physical exercise increases AMPK phosphorylation from a physiological perspective. Methodology: In this systematic review investigation, we searched literature databases such as MEDLINE-Pubmed, Web of Science, Scopus, and Science Direct. Papers published in the last five years that discussed AMPK, aerobic exercise, and high-intensity interval training met the inclusion criteria. A total of 103 published papers were found by utilizing the Web of Science, Pubmed, and Science Direct databases. For this systematic review, ten papers that met the inclusion criteria were selected and examined. In this study, the standard operating procedure was evaluated using the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA). Results: Exercise has been shown to increase AMPK phosphorylation. Discussion: High-intensity exercise has a higher potential to increase AMPK than aerobic exercise through increased AMP/ATP ratio, increased metabolic stress, and mitochondrial adaptation. AMPK activation has also been associated with increased fatty acid oxidation and glucose uptake by muscle. Conclusions: It can be concluded that physical exercise has been shown to consistently increase AMPK activation and has a positive impact on metabolic regulation. Thus, physical exercise is an effective intervention in improving metabolic function. Physical exercise has been shown to increase AMPK phosphorylation, thereby increasing GLUT4 translocation and increasing glucose uptake. © 2025 Federacion Espanola de Docentes de Educacion Fisica. All rights reserved.
Universitas Negeri Surabaya, Indonesia; STKIP PGRI Trenggalek, Indonesia; Universitas Negeri Jakarta, Indonesia; Bulacan State University, Philippines
