Moh Ali Ridho, Nining Widyah Kusnanik, Muhammad Ilham Mauluddin, Bambang Purwanto
Introduction: Muscle damage can be triggered by other free radicals formed during intense physical activites and the low response of antioxidants. The antioxidants that plays a role in responding to free radicals during physical activites is glutathione peroxidase. Objective: The main purpose of this study was to analyze the effects of high-intensity and moderate-intensity physical activities on muscle glutathione peroxidase (GPx) and serum troponin I levels as markers of damage. Methodology: The study was used a quantitative true experiment with a post-test only group design. This study was conducted at a total of 18 healthy mice with age of 8 weeks old male Balb/c mice, then divided into 3 groups: high-intensity physical activity (HI), moderate-intensity physical activity (MI), and control (CON) groups. The HI group performed running on a treadmill with a 90% of their maximum speed, while the MI group at 60%. Data was taken of 24 hours post-physical activity by taking blood samples to examine troponin I levels and skeletal muscle gastrocnemius homogenate to determine GPx activity. Results: the study found that the MI group had a higher concentration of GPx in the skeletal muscle gastrocnemius compared to the other control group. However, the concentration of troponin I showed significant differences between the groups, HI group had significantly higher levels of troponin I compared to the other groups. Conclusion: The high-intensity physical activity has the highest result in ncreasing Troponin I and the moderate intensity physical activity has the highest concentration of glutathione peroxidase compare to other groups. © 2025 Federacion Espanola de Docentes de Educacion Fisica. All rights reserved.
Universitas Airlangga, Indonesia; Universitas Negeri Surabaya, Indonesia
