Winda Azwani, Dini Aulia Cahya, Achmad Danny Gazali, Amira Puti Karima, Galuh Oktavya, Rahma Nur Istiqomah, Arleni Bustami, Faiza Aisya Rizqy, Puspita Anggraini Katili, Ujang Purnama, Mulyadi M. Djer
The human amniotic membrane (hAM) is a promising biomaterial in cardiac tissue engineering known for excellent viability, anti-inflammatory properties, and ability to support cellular adhesion. Its potential as a biomaterial, particularly after decellularization, offers a novel approach for myocardial regeneration in conditions such as cardiomyopathy and heart failure. Therefore, this study aimed to characterize the ultrastructure of fetal human cardiac fibroblasts-decellularized hAM (fHCFs-dehAM) using scanning electron microscopy (SEM), identify the functional groups of dehAM through fourier transform infrared (FTIR) spectroscopy, assess vimentin expression in fHCFs-dehAM via immunocytochemistry, and evaluate fHCF cell proliferation to determine cell viability on dehAM. In the process, hAM was successfully decellularized using 0.2% (w/v) trypsin/0.25% (w/v) ethylenediaminetetraacetic acid (EDTA) in phosphate-buffered saline (PBS), confirmed by the removal of the native epithelial layer through hematoxylin-eosin (H&E) staining and ultrastructural analysis. The results showed that fHCFs adhered to the basement membrane of the dehAM and retained the phenotype, as evidenced by the expression of the © 2025 Faculty of Engineering, Universitas Indonesia. All rights reserved.
Division of Pediatric Cardiology, RSAB Harapan Kita National Women and Children's Health Center, Jakarta, 11420, Indonesia; Faculty of Medicine, Universitas Negeri Surabaya, Surabaya, 60213, Indonesia; Cell, Tissue Culture, and Bio-imaging Division, Integrated Laboratory, Faculty of Medicine, Universitas Indonesia, Jakarta, 10430, Indonesia; Indonesian Medical Education and Research Institute (IMERI), Faculty of Medicine, Universitas Indonesia, Jakarta, 10430, Indonesia; Clinical Research Unit, RSAB Harapan Kita National Women and Children’s Health Center, Jakarta, 11420, Indonesia; Master’s Programme in Biomedical Science, Faculty of Medicine, Universitas Indonesia, Jakarta, 10430, Indonesia; Division of Biomedical Engineering, Department of Electrical Engineering, Faculty of Engineering, Universitas Indonesia, Depok, 16424, Indonesia; Healthcare Strategy Realization Office, Merck Healthcare, Darmstadt, 64293, Germany; Division of Pediatric Cardiology, Department of Pediatric, Faculty of Medicine, Universitas Indonesia, Dr. Cipto Mangunkusumo Hospital, Jakarta, 10430, Indonesia
