SYNTHESIS AND CHARACTERIZATION OF COPPER NANOPARTICLES USING CARICA DIENG (Carica pubescens) SEED EXTRACT AS A BIOREDUCER AND TESTING THEIR ANTIBACTERIAL ACTIVITY

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S. Sutoyo, A.P. Wardana, L. Yuanita, F. Setiani

2025 Rasayan Journal of Chemistry Vol. 18 Issue 2 Article Cited by 2 Quartile

Abstract

This study aims to create copper nanoparticles using a synthesis approach called the green synthesis method. In this method, copper sulfate pentahydrate (CuSO4.5H2O) is combined with Carica Dieng (Carica pubescens) seed extract, at 80oC, for 30 minutes. The materials were analyzed using techniques including UV-Vis spectrophotometer, FTIR, PSA, SEM, and XRD. The UV-Vis spectrum displayed absorption at a wavelength of 535 nm while FTIR analysis revealed a peak at 614 cm-1. That corresponds to Cu-O bond stretching. The Cu-NPs had a size of 14.49 nm, from the SEM morphology. The X-ray diffraction (XRD) analysis confirmed the presence of crystals with 2θ peaks at 31.77° and 44.66°. Antibacterial properties were tested using Staphylococcus aureus and Escherichia coli in a paper disc diffusion assay showing inhibition zones for both bacteria species. Cu-NPs demonstrated effects with a 15.84 mm zone against E. coli and moderate effects with a 9.84 mm zone, against S. aureus. © 2025, Rasayan Journal of Chemistry, c/o Dr. Pratima Sharma. All rights reserved.

Affiliations

Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Negeri Surabaya, East Java, Surabaya, Indonesia