Tukiran Tukiran, Andika Pramudya Wardana, Lilik Farkhiyah, Syahrul Lerry Hendrawan, Renita Meilasari
Objective: In vitro and in silico studies using α-glucosidase activity inhibition aimed at identifying the potential antidiabetic properties of ethanol extracts of D. virgatus leaves. Material and Method: Compounds in the ethanol extract of D. virgatus leaves were identified by LC-MS. In vitro and in silico methods were used to determine the antidiabetic activity. Result and Discussion: The protein codes (PDB ID) used were 3AJ7 and 3W37, then acarbose was used as a positive control. By using LC-MS, 160 compounds were identified from the extract. All of the identified compounds were tested in silico. There are 18 compounds (with 3AJ7 protein) and 10 compounds (with 3W37 protein) that fulfill Lipinski's rule with binding affinity values lower than acarbose, namely ≤-8.2 kcal/mol for 3AJ7 protein and ≤-8.1 kcal/mol for 3W37. In addition, α-glucosidase inhibition was used to assess the antidiabetic effect of the ethanol extract of D. virgatus leaves in vitro, resulting in IC50 values of 144.11 ppm for the extract and 3.78 ppm for acarbose. The IC50 values of the extract were included in the medium category. © 2025, University of Ankara. All rights reserved.
Universitas Negeri Surabaya, Faculty of Mathematics and Natural Science, Department of Chemistry, Surabaya, 60231, Indonesia
